D4034, DNA Clean & Concentrator™-25 (Capped) (200 Preps)
About DNA Clean & Concentrator™-25
The DNA Clean & Concentrator™-25 (DCC™-25) is designed for rapid desalting and purification of up to 25 µg DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin™ column. The product featuresFast-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
|DNA Recovery||Typically, up to 25 µg total DNA can be eluted into as little as 25 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.|
|DNA Size Limits||50 bp to 23 kb|
|Sample Sources||DNA from PCR, restriction endonuclease digestions, plasmid preparations, kinase reactions, etc.|
|DNA Purity||High-quality, purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.|
|Product Detergent Tolerance||≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS|
The DNA Clean & Concentrator™ yields high quality DNA for efficient transcription reactions. Lanes: M: 1 kb Marker; A: DNA template purified using the DNA Clean & Concentrator™; B: a 7 kb RNA transcript generated in vitro from A.