D5001-5, M-Desulphonation Buffer (10 ml)
Streamlined, proven procedure for bisulfite conversion of DNA.Desulphonation and recovery of bisulfite-treated DNA with a spin column.Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
The EZ DNA Methylation™ Kit features a simplified procedure that streamlines bisulfite treatment of DNA. The kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. The product's innovative in-column desulphonation technologies eliminate otherwise cumbersome precipitations. The kit is designed to reduce template degradation, minimize DNA loss during treatment and cleanup, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. This kit is validated for use with Illumina's GoldenGate and Infinium Assays.