ß-Agarase is an enzyme that will completely digest the polysaccharide backbone of molten agarose into alcohol soluble oligosaccharides. DNA electrophoresed in low melting temperature agarose gels (e.g., SeaPlaqueTM, SeaPlaque GTGTM, or NuSieveTM GTG Agarose) can be recovered quantitatively after the gel is melted and then digested with this enzyme. Any remaining agarose oligosaccharides will not gel or interfere with subsequent DNA manipulations such as cloning, labeling, restriction digestion, or sequencing.
The enzyme is stable when stored at -20°C.
ß-Agarase is supplied at 1,000 units/ml in a buffer consisting of 50% glycerol, 50 mM Tris/HCl, 100 mM NaCl, and 0.1% Triton® X-100 at pH 7.5.
One unit of ß-Agarase is defined as the amount of enzyme necessary to completely digest 200 mg of a molten 1% SeaPlaque GTG Agarose gel prepared in 40 mM Bis Tris/HCl, 40 mM NaCl, 1 mM EDTA (pH 6.0) at 40°C in 1 hour.