1ml Serological Pipette, Filter, Sterile, Ind. Wrap,  Yellow,  500 pcs/pk

1ml Serological Pipette, Filter, Sterile, Ind. Wrap, Yellow, 500 pcs/pk

08-15-00001, 5x HOT FIREPol Probe qPCR Mix Plus (NO ROX), 1 ML, 250 x 20 µL Rxns

5x HOT FIREPol® Probe qPCR Mix Plus (no ROX)

For in vitro use only

Description:

HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative  PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. 


HOT FIREPol®DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

TVA inclus
226,10 RON
Cod SKU (T)
08-15-00001

Applications:

- Detection and quantification of DNA and cDNA targets 

- Profiling gene expression 

- Microbial detection 

- Viral load determination

Mix Compatibility:

Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon® 2 & MiniOpticon®

Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000

Eppendorf: Mastercycler®: ep realplex2 & ep realplex4   

Illumina: The Eco™   

Roche: LightCycler® 480

Mix Composition:

- HOT FIREPol® DNA Polymerase

- 5x Probe qPCR buffer

- 15 mM MgCl2 1x PCR solution – 3 mM   

- dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP  

- No ROX dye

Shipping and Storage Conditions:

Routine storage: -20ºC 
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® Probe qPCR Mix Plus (no ROX).

 

Mastercycler is a trademark of Eppendorf AG. Rotor-Gene is a registered trademark of Corbett Research (Qiagen). CFX96, CFX384, iQ, MyiQ, Chromo4, Opticon 2, MiniOpticon are trademarks of Bio-Rad Laboratories. LightCycler is a registered trademark of Roche Applied Sciences. The Eco is registered trademark of Illumina Inc.