ZymoBIOMICS™ DNA/RNA Mini Kit
About ZymoBIOMICS™ DNA/RNA Mini Kit
The ZymoBIOMICS™ DNA/RNA Mini Kit is designed for purifying DNA and RNA from a wide array of sample inputs (e.g. feces, soil, plant, water, and biofilms) that is ready for microbiome or metagenome analyses. The ZymoBIOMICS™ innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. gram negative/positive bacteria, fungus, protozoans, and algae). The provided DNA/RNA Shield™ preserves nucleic acids at ambient temperatures, providing an unbiased molecular snapshot of the sample. The procedure uses Zymo-Spin® column technology that results in high-quality DNA and total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors (e.g. polyphenols, humic acids, and fulvic acids) and is ready for RT-PCR, arrays, sequencing, etc.
|Equipment||Microcentrifuge, vortex, cell disrupter (recommended).|
|Sample Sources||Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal/bacterial cells, biofilms, and water.|
|Storage||DNA and RNA eluted with DNase/RNase-Free Water can be stored at ≤70ºC. The addition of RNase inhibitors in highly recommended for prolonged storage.|
|Purity||High quality DNA and RNA (A260/A280 >1.8, A260/A230 >1.8) are recovered.|
|Size Limit||Capable of recovering DNA and total RNA ≥17 nucleotides.|
|Recovery||The DNA/RNA binding capacity of the Zymo-Spin™ IIICG Column is ~100 µg.|
|Sample Preservation||DNA/RNA Shield™ lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures.|
|Bead Beating System||ZymoBIOMICS™ innovative lysis system ensures complete homogenization of the microbial cell walls and accurate microbial analysis, free of bias.|
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