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ZymoBIOMICS™-96 DNA Kit

ZymoBIOMICS™-96 DNA Kit

 

 Rapid, robust, and simple high throughput spin column purification of high quality, inhibitor-free DNA from any sample including feces, soil, water, biofilms, swabs, saliva, body fluids, etc. in a highly concentrated, low elution volume format for low bio
 The ZymoBIOMICS™ innovative lysis system enables efficient and unbiased lysis of microbes including Gram-positive and negative bacteria, fungus, protozoans, algae, and viruses.
 Unbiased extraction of ultra-pure DNA makes the ZymoBIOMICS™-96 DNA Kit ideal for 16S rRNA gene sequencing, shotgun metagenomic sequencing, arrays, PCR and other sensitive applications.
 
ProductSizeCatalog #
ZymoBIOMICS™-96 DNA Kit (Includes ZR BashingBead™ Lysis Rack) 2x96 Preps D4303
ZymoBIOMICS™ 96 DNA Kit (Lysis Matrix Not Included) 2x96 preps D4307
ZymoBIOMICS™-96 DNA Kit (Includes ZR BashingBead™ Lysis Tubes) 2x96 preps D4309

 

About ZymoBIOMICS™-96 DNA Kit

The ZymoBIOMICS™-96 DNA Kit is designed for purifying DNA from a wide array of sample inputs (e.g. feces, soil, water, and biofilms), that is immediately ready for microbiome or metagenome analyses.  The ZymoBIOMICS innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae)1 making it ideal for microbial community profiling. Unbiased mechanical lysis of tough microbes is achieved by bead beating with the innovative ZymoBIOMICS ultra-high density BashingBeads and validated using the ZymoBIOMICS Microbial Community Standard2, as shown in Figure 3.  In addition, the ZymoBIOMICS™-96 DNA kit is equipped with Zymo’s Proprietary OneStep PCR Inhibitor Removal technology enabling PCR from the most PCR prohibitive environmental samples rich in humic and fulvic acids, tannins, melanin, and other polyphenolic compounds. Coupling state-of-the-art lysis technology with Zymo-Spin purification technology results in superior yields of ultra-pure DNA ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing.

  

DNA Recovery Up to 5 μg total DNA can be eluted into 10 μl (6 μl minimum) ZymoBIOMICS™ DNase/RNase Free Water.
Equipment Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer
Sample Sources Bacterial, fungal, viral and other microbial DNA can be isolated from 5-20 mg (wet weight) of fungal/bacterial cells
DNA Purity High quality, inhibitor-free DNA is eluted with ZymoBIOMICS™ DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing.
Bead Beating System The ZymoBIOMICS™ innovative lysis system enables complete homogenization/disruption of the microbial cells walls and accurate microbial DNA analysis, free of bias. To ensure unbiased lysis it is recommend that the ZymoBIOMICS™ Microbial Community Standard is used for calibration of each bead beating device
DNA Integrity Generally, post bead beating, genomic DNA has an average size of 15-20 kb depending on the initial quality of the sample making it amenable to Next-Gen sequencing platforms requiring high molecular weight DNA. For optimal DNA integrity, collect samples in DNA/RNA Shield™.
Bioburden A single preparation is guaranteed to contain less than 3 bacterial genomic copies per 1 μl of eluate as determined by quantitative amplification of the 16S rRNA gene when eluted using 100 μl water

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6 Articol(e)

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