Bine ați venit la magazinul online BioZyme!

Cosul meu:

0item(s)
Nu aveti nici un produs in cosul de cumparaturi

0

×

Registration

Profile Informations

Login Datas

or login

First name is required!
Last name is required!
First name is not valid!
Last name is not valid!
This is not an email address!
Email address is required!
This email is already registered!
Password is required!
Enter a valid password!
Please enter 6 or more characters!
Please enter 16 or less characters!
Passwords are not same!
Terms and Conditions are required!
Email or Password is wrong!

ZymoPURE-EndoZero Plasmid Maxiprep Kit

ZymoPURE-EndoZero™ Plasmid Maxiprep Kit

 The fastest spin-column based procedure for purifying up to 1.2 mg of endotoxin-free plasmid DNA.
 Routinely achieve endotoxin levels of ≤ 0.025 EU/µg of plasmid DNA in 20 minutes.
 Plasmid DNA that is ideal for transfection in sensitive cells and in vivo research.
 
ProductSizeCatalog #
ZymoPURE EndoZero™ Plasmid Maxiprep 10 Preps D4205


About ZymoPURE-EndoZero™ Plasmid Maxiprep Kit

The ZymoPURE EndoZero Plasmid Maxiprep kit features a spin column-based method for the purification of up to 1.2 mg of high-quality endotoxin-free plasmid DNA in less than 20 minutes. The isolated plasmid DNA is ready for immediate use, and does not require subsequent precipitation steps.

ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl).  In addition, the kit incorporates our unique EndoZero spin-columns to reduce endotoxin levels down to 0.025 EU/µg of plasmid DNA without lengthy incubations, gravity flow anion-exchange columns, expensive chromatography columns, or time-consuming centrifugation steps.  The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, in vivo studies, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.

As an added convenience, the ZymoPURE-EndoZero Plasmid Maxiprep contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.

 

Processing Time 20 min
Equipment Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge.
DNA Purity Eluted DNA is ultrapure, endotoxin-free, and well suited for transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, and other sensitive applications. Typical Abs260/280 ≥ 1.8 and Abs260/230 ≥ 2.0. Endotoxin levels ≤ 0.025 EU/µg of plasmid DNA.
Plasmid DNA Size Up to 25 kb
Plasmid DNA Yield Up to 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)
Recovery Volume ≥ 200 µl of ZymoPURE™ Elution Buffer or DNase free water

 

2 Articol(e)

Tabel  Lista 

2 Articol(e)

Tabel  Lista 

Newsletter
Follow us