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ChIP-seq

ChIP-seq

 

Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomic researches, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.


  1. Chromatin preparation: cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.
  2. Chromatin shearing: fragmentation of chromatin by sonication down to desired fragment size (100-500 bp)
  3. Chromatin IP: protein-DNA complexe capture using specific ChIP-seq grade antibodies against the histone or transcription factor of interest
  4. DNA purification: chromatin reverse cross-linking and elution followed by purification
  5. NGS Library Preparation: adaptor ligation and amplifcation of IP'd material
  6. Bioinformatic analysis: read filtering and trimming, read specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc.

Need guidance?

Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.

 

Made for ChIP-seq

 
Cat. No.ProductFormat 
C01010171 Auto iDeal ChIP-seq kit for Histones 
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ...
100 rxns  
C01010172 Auto iDeal ChIP-seq Kit for Transcription Factors 
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. U...
100 rxns  
C01010140 Auto True MicroChIP kit 
The Auto True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. This m...
16 rxns  
C01010153 Auto Universal Plant ChIP-seq kit 
The Auto Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including  Arabidopsis, ma...
24 rxns  
B01060001 Bioruptor® Pico sonication device 
The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system optimized for shearing micro-...
1 unit  
C01019027 ChIP Cross-link Gold 
Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.However, formaldehyde is usually not effective to cross-li...
600 µl  
C01010051 iDeal ChIP-seq kit for Histones 
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ChIP prior...
24 rxns  
C01010055 iDeal ChIP-seq kit for Transcription Factors 
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. U...
24 rxns  
B03000002 IP-Star® Compact Automated System 
The IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental cond...
1 unit  
C01010130 True MicroChIP Kit 
The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. T...
16 rxns  
C01010152 Universal Plant ChIP-seq kit 
The Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including  Arabidopsis, maize, ...
24 rxns  
C30010014 2 ml microtubes for SX-8G IP-Star® Compact 
These tubes are intended for being used on the SX-8G IP-Star® Compact Automated System.
100 pc  
C30080030 96 well microplates for IP-Star® 
Standard 96 well transparent plates made by polyestyrene and compatible with automated systems. Solid U-bottom plates free of DNAase and RNAase and human DNA.
10 pc  
C03010010 Auto IPure kit v2 
The DNA recovery after ChIP or MeDIP might be an issue for specific downstream applications (e.g. Next generation sequencing). Diagenode's IPure kit is the o...
100 rxns  
C02020011 Auto MethylCap kit 
The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methyl binding domain) protein . The Diagenode’s Auto MethylCa...
48 rxns  
C01010151 Auto Plant ChIP-seq kit 
Diagenode's Plant ChIP-seq Kit is the only validated and complete solution for obtaining high quality ChIP-seq results from plant material. Specifically deve...
24 rxns  
C01010141 Auto True MicroChIP & MicroPlex Library Preparation™ Package 
The Auto True MicroChIP kit in combination with the MicroPlex Library Preparation kit allows for performing ChIP-seq on as few as 10,000 cells. Thi...
16 ChIP rxns and 12 library prep rxns  
C01010053 iDeal ChIP-seq for Histones & Library Preparation Package (incl. Index Primer Set 1) 
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ChIP prior...
24 rxns  
C01010056 iDeal ChIP-seq for Transcription Factors & MicroPlex Library Preparation™ Package 
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. Unlike comp...
10 ChIP and 12 library prep rxns  
C01010173 Immunoprecipitation Buffers (iDeal ChIP-seq kit for Histones) 
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker).
24 rxns  
C01010174 Immunoprecipitation Buffers (iDeal ChIP-seq kit for TFs) 
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker).
24 rxns  
C03010015 IPure kit v2 
High DNA recovery after ChIP or MeDIP is critical for specific downstream applications (e.g. Next generation sequencing). Diagenode's IPure kit is the only D...
100 rxns  
C17040004 Maize B73 inner stem ZmB1-UTR primer pair 
This primer pair specifically amplifies a genomic region in the 5’-UTR of the B1 gene from Maize (Zea mays clone B73). The primers are thoroughly teste...
50 µl  
C17040005 Maize B73 inner stem ZmCopia primer pair 
This primer pair specifically amplifies a genomic region in the Copia gene from Maize (Zea mays clone B73). The primers are thoroughly tested in conjunction ...
50 µl  
C01010150 Plant ChIP-seq kit 
Diagenode's Plant ChIP-seq Kit is the only validated and complete solution for obtaining high quality ChIP-seq results from plant material. Specifically deve...
24 rxns  
C17040011 Poplar xylem PtrCopia-orth primer pair 
This primer pair specifically amplifies a genomic region from a sequence orthologous to the Maize Copia gene from poplar (Populus trichocarpa clone Nisqually...
50 µl  
C17040010 Poplar xylem PtrMYBTF1 primer pair 
This primer pair specifically amplifies a genomic region from a gene coding a MYB transcription factor in poplar (Populus trichocarpa clone Nisqually). The p...
50 µl  
C17040009 Rice seedlings OsMADS6 primer pair 
This primer pair specifically amplifies a genomic region from the MADS6 gene from rice (Oriza sativa ssp Japonica cv. Nipponbare). The primers are thoroughly...
50 µl  
C17040006 Tomato leaves SlChr2-reg8 primer pair 
This primer pair specifically amplifies a genomic region from chromosome 2 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ...
50 µl  
C17040007 Tomato leaves SlChr4-NC1 primer pair 
This primer pair specifically amplifies a genomic region from chromosome 4 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ...
50 µl  
C01010131 True MicroChIP & MicroPlex Library Preparation™ Package 
The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. This microC...
16 ChIP rxns and 12 library prep rxns  
 
 

18 Articol(e)

Tabel  Lista 

18 Articol(e)

Tabel  Lista 

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