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CHIP-QPCR

ChIP-qPCR

 

Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.



  1. Chromatin preparation: cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.
  2. Chromatin shearing: fragmentation of chromatin by sonication down to desired fragment size (100-500 bp)
  3. Chromatin IP: protein-DNA complexe capture using specific ChIP-grade antibodies against the histone or transcription factor of interest
  4. DNA purification: chromatin reverse cross-linking and elution followed by purification
  5. qPCR and analysis: using previously designed primers to amplify IP'd material at specific loci

Need guidance?

Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.

 

Made for ChIP-qPCR

Cat. No.ProductFormat 
C01010171 Auto iDeal ChIP-seq kit for Histones 
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ...
100 rxns  
C01010172 Auto iDeal ChIP-seq Kit for Transcription Factors 
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. U...
100 rxns  
C01010140 Auto True MicroChIP kit 
The Auto True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. This m...
16 rxns  
C01010153 Auto Universal Plant ChIP-seq kit 
The Auto Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including  Arabidopsis, ma...
24 rxns  
C01019027 ChIP Cross-link Gold 
Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.However, formaldehyde is usually not effective to cross-li...
600 µl  
C01010051 iDeal ChIP-seq kit for Histones 
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ChIP prior...
24 rxns  
C01010055 iDeal ChIP-seq kit for Transcription Factors 
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. U...
24 rxns  
B03000002 IP-Star® Compact Automated System 
The IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental cond...
1 unit  
C01010130 True MicroChIP Kit 
The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. T...
16 rxns  
C01010152 Universal Plant ChIP-seq kit 
The Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including  Arabidopsis, maize, ...
24 rxns  
C02020011 Auto MethylCap kit 
The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methyl binding domain) protein . The Diagenode’s Auto MethylCa...
48 rxns  
C01010141 Auto True MicroChIP & MicroPlex Library Preparation™ Package 
The Auto True MicroChIP kit in combination with the MicroPlex Library Preparation kit allows for performing ChIP-seq on as few as 10,000 cells. Thi...
16 ChIP rxns and 12 library prep rxns  
B01020001 Bioruptor® Plus sonication device 
The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (wate...
1 unit  
C30080031 ChIPettor™ 96 Well Plate 
Our ChIPettor™ 96 deep well plates are compatible with both the ChIPettor™ System for Histones and ChIPettor™ System for Transcription Fact...
5 pc  
C01010160 ChIPettor™ System for Histones x10 
The ChIPettor™ System for histones has everything you need for high-quality, fast, and easy chromatin immunoprecipitation: a full set of reagents for...
10 rxns  
C01010162 ChIPettor™ System for Histones x24 
The ChIPettor™ System for histones has everything you need for high-quality, fast, and easy chromatin immunoprecipitation: a full set of reagents for...
24 rxns  
C01010161 ChIPettor™ System for Transcription Factors x10 
The ChIPettor™ System for Transcription Factors has everything you need for high-quality, fast, and easy chromatin immunoprecipitation: a full set of...
10 rxns  
C01010163 ChIPettor™ System for Transcription Factors x24 
The ChIPettor™ System for Transcription Factors has everything you need for high-quality, fast, and easy chromatin immunoprecipitation: a full set of...
24 rxns  
C30040010 ChIPettor™ Tips Protein A 
Our ProA ChIPettor™ tips contain a packed-resin bed that keeps the ProA affinity resin contained within the tip end. The tips simplify the capture, pur...
12 tips/strip  
C03030001 DNA co-precipitant 
This IP grade product is optimized to be used as carrier for DNA purification after chromatin immunoprecipitation (ChIP) and after methylated DNA immunopreci...
100 µl  
C03030002 DNA precipitant 
This IP grade product is used for the precipitation of DNA after chromatin immunoprecipitation (ChIP) and after methylated DNA immunoprecipitation (Methyl DN...
1 ml  
C03080030 DNA purifying slurry 
The DNA purifying slurry can be used for easy and fast DNA purification and provides you with DNA suitable for qPCR analysis. If you need DNA of higher purit...
10 ml  
C01010120 Elution Module 
Optimized for for the elution of immunoprecipitated DNA bound to magnetic or agarose beads.
1 unit  
C06060010 GenDNA RNAse (DNAse-free) 
Our RNAse is DNAse-free. No boiling treatment is needed; it can be used directly from the vial. This product is particularly well suited for the use in methy...
10 µl  
C01010063 HighCell# ChIP kit protein G 
Perform ChIP with up to 10 million cells per IP. Recover more DNA and limit bias downstream applications.
48 rxns  
C01010062 HighCell# ChIP kit protein A 
Perform ChIP with up to 10 million cells per IP. Recover more DNA and limit bias downstream applications.
48 rxns  
C01010100 Histone ChIP kit 
Perform robust ChIP (Chromatin Immunoprecipitation) on 100.000 cells per IP. *Number of cells that can be lysed and sheared with this kit.
18 rxns  
C01010053 iDeal ChIP-seq for Histones & Library Preparation Package (incl. Index Primer Set 1) 
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ChIP prior...
24 rxns  
C01010056 iDeal ChIP-seq for Transcription Factors & MicroPlex Library Preparation™ Package 
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. Unlike comp...
10 ChIP and 12 library prep rxns  
C01010173 Immunoprecipitation Buffers (iDeal ChIP-seq kit for Histones) 
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker).
24 rxns  
C01010174 Immunoprecipitation Buffers (iDeal ChIP-seq kit for TFs) 
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker).
24 rxns  
C01010072 LowCell# ChIP kit protein A 
The Diagenode’s LowCell# ChIP kit is the only complete magnetic bead based ChIP (Chromatin Immunoprecipitation) kit that can be used with as low as 100...
48 rxns  
C01010073 LowCell# ChIP kit protein G 
The Diagenode’s LowCell# ChIP kit is the only complete magnetic bead based ChIP (Chromatin Immunoprecipitation) kit that can be used with as low as 100...
48 rxns  
C17040004 Maize B73 inner stem ZmB1-UTR primer pair 
This primer pair specifically amplifies a genomic region in the 5’-UTR of the B1 gene from Maize (Zea mays clone B73). The primers are thoroughly teste...
50 µl  
C17040005 Maize B73 inner stem ZmCopia primer pair 
This primer pair specifically amplifies a genomic region in the Copia gene from Maize (Zea mays clone B73). The primers are thoroughly tested in conjunction ...
50 µl  
C09010010 MethylTaq DNA polymerase 
MethylTaq DNA polymerase is a high-performance Hot Start thermostable recombinant DNA polymerase. MethylTaq is an extremely robust modified Taq DNA polymeras...
250 units  
C03040001 MicroChIP DiaPure columns 
Diagenode's MicroChIP DiaPure columns are ideal in combination with the True MicroChIP kit allowing for the purification and elution of ChIP'd DNA in volumes...
50 rxns  
C01010081 OneDay ChIP kit 
Perform ChIP (Chromatin Immunoprecipitation) on large cell numbers. Check out our ChIP-Seq grade antibodies
180 rxns  
C15410048 p53K372me1 polyclonal antibody - Classic 
Alternative names: TP53, P53, TRP53, LSF1 Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), methylated at K372, using a KLH-conjug...
50 µg/25 µl  
C01010150 Plant ChIP-seq kit 
Diagenode's Plant ChIP-seq Kit is the only validated and complete solution for obtaining high quality ChIP-seq results from plant material. Specifically deve...
24 rxns  
C17040011 Poplar xylem PtrCopia-orth primer pair 
This primer pair specifically amplifies a genomic region from a sequence orthologous to the Maize Copia gene from poplar (Populus trichocarpa clone Nisqually...
50 µl  
C17040010 Poplar xylem PtrMYBTF1 primer pair 
This primer pair specifically amplifies a genomic region from a gene coding a MYB transcription factor in poplar (Populus trichocarpa clone Nisqually). The p...
50 µl  
C17040008 Rice seedlings OsChr4-reg9 primer pair 
This primer pair specifically amplifies a genomic region from chromosome 4 from Rice (Oriza sativa ssp Japonica cv. Nipponbare). The primers are thoroughly t...
50 µl  
C17040009 Rice seedlings OsMADS6 primer pair 
This primer pair specifically amplifies a genomic region from the MADS6 gene from rice (Oriza sativa ssp Japonica cv. Nipponbare). The primers are thoroughly...
50 µl  
C17040006 Tomato leaves SlChr2-reg8 primer pair 
This primer pair specifically amplifies a genomic region from chromosome 2 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ...
50 µl  
C17040007 Tomato leaves SlChr4-NC1 primer pair 
This primer pair specifically amplifies a genomic region from chromosome 4 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ...
50 µl  
C01010090 Transcription ChIP Kit 
form robust ChIP (Chromatin Immunoprecipitation) on large cell numbers. *Number of cells that can be lysed and sheared with this kit. >> Check out...
18 rxns  
C01010131 True MicroChIP & MicroPlex Library Preparation™ Package 
The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. This microC...
16 ChIP rxns and 12 library prep rxns  
C03030020 XL GenDNA Extraction Module 
This module has been designed for the extraction of 60 µg of DNA from culture cells.
60 rxns  
    
 
 
 
 

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